- Cut the plant at its base beneath the twist tie.
- Roll the bag between your hands and use your fingers to break apart the panicles, to release the seeds.
- Undo the twist tie and remove the plants from the bag. You will be left with a large bag with a lot of tiny seeds at the bottom. To make pouring the seeds into the envelope easier, you can cut a slit in the bag.
- Place the seeds into a clean seed envelope for shipment back to DDPSC.
- Place the seeds into a clean seed envelope and label Setaria viridis, family number, plant number, as well as the date the plant was planted (P) and seeds were collected (H, for harvested). If that plant displayed a mutant phenotype, write that on the envelope too. For mutant plants, include the generation. This should start with an M, and the number should be 1 greater than the seeds you planted, since this is the next generation. (i.e. if you planted M4 seeds, then on your seed packet of seeds collected from that plant, you would write M5). THE SEEDS CANNOT BE USED IF YOU FORGET THIS STEP!!! For your convenience, labels have been printed. All you need to do is stick the label to your seed envelope and write in the plant and harvest date as well as any mutant phenotypes displayed by that plant.
Example seed packets:
S. viridis 00317 M5 #2
Phen: Light Green
P: 1/21/15 H: 5/2/15